Costimulation of T cells by plastic-immobilized B7-1 IgG.
Resting T cells isolated from a healthy donor (A), T cells from a healthy donor preactivated with PHA (B), and PHA-preactivated remission T cells from patient no. 39 (AML-M5a) (C) were cultured in 96-well plates (5 × 104 cells per well) coated with CD8 IgG (■) or B7-1 IgG (▪, 75 to 100 ng per well) and the indicated concentrations of OKT3. 3H-thymidine-uptake was determined after 72 hours of culture as described in “Materials and methods.” Data are expressed as mean ± SD of triplicate wells. T cells alone: 0.3 ± 0.1 cpm × 103 (A); 2.9 ± 0.8 cpm × 103 (B); 0.3 ± 0.03 cpm × 103(C). Asterisks indicate P = .05 values by one-sided Wilcoxon tests for unpaired rank sums compared with CD8 IgG–induced proliferation.
