RLGS mixing gels used in cloning procedure.

Normal PB genomic DNA (panel A) with no additionalNotI-EcoRV clone. PB with 5.2 pg (panel B) and 20.8 pg (panel C) of the radiolabeled candidate clone added to the genomic DNA. Progressive enhancement of the RLGS fragment of interest confirms identification of the correct NotI-EcoRV clone.