Southern blots showing methylation of RLGS clones in AML cell lines.

Lane 1: Control normal donor peripheral blood (PB) is digested withEcoRV only (RV only) to show size of unmethylated band. Lanes 2-6: PB and AML cell line DNA is double-digested withEcoRV and methylation-sensitive NotI (RV-NotI). The presence of an uncut band in these lanes is indicative of methylation at the NotI site. Blots are probed with the RLGS clones 2C40 (panel A), 5C08 (panel B), and 3B36 (panel C). The 1.7-kilobase (kb) band in (panel B) is due to partial methylation of an internal NotI site within the larger 3-kbEcoRV-NotI fragment. Kas-1 indicates Kasumi-1.