IL-11 does not affect DC maturation mediated by CD40L, TNF-α, and LPS.

DCs cultured for 6 to 7 days with GM-CSF and IL-4 (A) were further incubated with maturation stimuli in the presence and absence of IL-11 (B). Overlay diagrams show the expression of the relevant antigens versus negative controls. Addition of CD40L, TNF-α, or LPS to immature DCs induced a phenotypic pattern typical of terminally differentiated elements: CD1a⁻, HLA-DR⁺, CD80⁺, CD86⁺, and CD83⁺. Addition of IL-11 (gray line) did not modify the DC profile as regards mean fluorescence intensity (MFI) or the percentage of positive cells.