IL-11 inhibits cytokine production by activated monocytes but not by DCs.

DCs were matured with murine L cells stably transfected with CD40L in the presence (▪) or absence (■) of IL-11. Supernatants were collected after 40 hours of coculture and assayed for IL-12 (p75) (A), IL10, TNF-α, and IL-1β concentration (B). CD14⁺ cells were stimulated for 16 hours with IFN-γ and then incubated with LPS. After 24 hours, cells were collected and supernatants were assayed for IL-12 (A). IL-11 reduced IL-12 production by activated monocytes from a mean value of 780 pg/mL to 100 pg/mL (P < .05). No effect was seen on DCs as regards IL-12 (A), IL10, TNF-α, or IL-1β secretion (B).