Fig. 5.
Metabolic labeling of the wild-type and mutant rXIIIBs.
Radiolabeled cell lysate and culture medium, harvested at 0 hour after labeling, were immunoprecipitated with an anti-XIIIB antibody, followed by 10% SDS-PAGE under nonreducing or reducing conditions, with or without β-mercaptoethanol (β-ME).