Pulse-chase experiments for rXIIIBs.

Experiments were carried out identically to those in Figure 5, except that radiolabeled samples were taken at various time intervals and electrophoresed under reducing conditions (A). The intensity of the band for cell lysates harvested at 0 hour was defined as 100%, and the relative intensity of each band was plotted (B). Open and closed circles show cell lysates and culture media of the wild type, respectively. Open and closed squares depict cell lysates and culture media of the delG mutant, respectively.