Intracellular localization of wild-type and mutant rXIIIBs.

Immunocytochemical staining with an anti-XIIIB antibody and an anti-Golgi 58K protein (Golgi58) or anti-PDI antibody was carried out using BHK cells expressing wild-type (WT) or mutant rXIIIB (delG). The samples were examined by an epifluorescence microscope × 400 (A). Electron microscopic immunolocalization of rXIIIB was also performed using an anti-XIIIB antibody and ultrathin sections of infected BHK cells (B). Boxed areas of pictures on the left (× 3000) were reexamined at a magnification of × 12 000 (right).