Cyclin D2 expression is NF-κB dependent.

(A) NF-κB binding activity. Whole-cell extracts of H/RS cells and control cells were analyzed by EMSA using an H2K binding site probe for NF-κB. (B) Expression of cyclins in H/RS and control cells. Steady state levels of cyclin D1, cyclin D2, and cyclin E proteins were determined by Western blotting using specific antibodies, as indicated (see “Materials and methods”). (C) Cyclin D2 expression of Ad5-control– and Ad5-IκBΔN–infected L428 cells. Whole-cell extracts of noninfected and infected L428 cells were prepared at the indicated time points and analyzed by Western blotting with anticyclin D2 antibody (upper panel). The blot was stripped and reprobed with anti-CDK4 antibody (lower panel). NI indicates noninfected.