CD40 and CD86 are NF-κB–dependent target genes.

(A) L428 cells were infected with Ad5-control and Ad5-IκBΔN. RNA was analyzed using Atlas cDNA Array I (Clontech Laboratories). The figure shows a graphic representation of mRNA expression ratios between Ad5-control– and Ad5-IκBΔN–infected cells. (B,C) Total RNA was extracted 24 hours after infection from L428 cells, infected with Ad5-control (L428c24) or Ad5-IκBΔN (L428i24), and from different control and H/RS cell lines. Northern blots were probed with³²P-labeled CD40 or CD86 cDNA probes. As a control, the stripped blot was reprobed with a ³²P-labeled GAPDH cDNA probe. (D,E) Flow cytometric analysis of surface CD40 or CD86 expression on L428 cells 48 hours after infection with Ad5-control (L428c48) and Ad5-IκBΔN (L428i48) (left panels). Flow cytometric analysis of surface CD40 or CD86 expression on control, various H/RS, and infected L428 cells. The graph represents peak fluorescence intensities of the indicated cell lines (right panels).