Trx80 induces proliferation of CD14⁺monocytes.

(A) Purified CD14⁺ monocytes (> 94% purity) were stimulated with Trx, Trx80, or IL-2 and grown in AIM V medium in triplicate wells for 3 days. Twelve hours before harvesting, 0.5 μCi³H-thymidine was added. The mean ³H-thymidine incorporation from 7 donors is shown with black dots and the median values are indicated with black bars. There was a significant increase in thymidine incorporation in cultures stimulated with 100 nM and 1 μM Trx80 compared to the unstimulated cells, which is indicated withP values in the figure. (B) Purified CD14⁺monocytes were grown in AIM V medium and stimulated with 1 μg/mL PMB, Trx80, and LPS alone, or PMB together with either 1 μM Trx80 or 10 ng/mL LPS for 3 days. Twelve hours before harvesting 0.5 μCi³H-thymidine was added. Error bars indicate SDs of triplicate cultures. The figure depicts one representative experiment of 3.