Sorting regions used for preparation of Lin⁻c-kit⁺Sca-1⁺CD38⁻and Lin⁻c-kit⁺Sca-1⁺CD38⁺cell populations.

The Lin⁻ cells from normal mice were enriched for c-kit⁺Sca-1⁺ cells using the R1 window and sorted on the basis of CD38 expression using R2 and R3 sorting gates. The cells from 5-FU–treated mice were prepared using R4, R5, and R6 gates. The experiments were performed with NIM-R5 anti-CD38 antibody (Southern Biotechnology Associates).