Effect of several candidate peptides synthesized based on the screening results of the PS-SPCLs with regard to superoxide generation in human monocytes.

(A) Human monocytes were stimulated with 1 μM concentrations of several peptides, 1 μM fMLF, or 100 nM phorbol myristate acetate, and the generated superoxide was measured using cytochrome c reduction assay. (B) The concentration dependency of the superoxide generation induced by peptides in monocytes. HFYLPM, ▪; MFYLPM, ○; HFYLPm, ▴; LFMYHP, ▿. (C) Time course of the peptide-induced superoxide generation in human monocytes; 3.3 μM of HFYLPM and 330 nM of HFYLPm were used. The results are presented as means ± SE of 3 independent experiments. *P < .01 versus vehicle treatment. Medium, ■; HFYLPM, ▪; MFYLPM, ○; HFYLPm, ▴.