Fig. 4.
![Fig. 4. Cell specificity of peptides. / Prepared human primary monocytes, neutrophils, and cultured HL60, Jurkat, U937, NIH3T3, 3Y1, 3T3L1, and PC12 cells were loaded with fura-2 and stimulated with effective concentrations of the 3 peptides (1 μM for monocytes and neutrophils; 10 μM for HL60 and Jurkat; 20 μM for U937, NIH3T3, 3Y1, 3T3L1, and PC12). (A) The peak level of the [Ca++]i increase was recorded. (B) Monocytes and neutrophils were stimulated with 10 μM of each peptide. Superoxide generation was measured using cytochrome c reduction assay. Data are presented are means ± SE from at least 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2854/6/h80910966004.jpeg?Expires=1735564791&Signature=3N0xpn~HOHc0l04IWKSP8Sz6MkoppVdJx-iQq1O3Kvylq9l5bzFrdoHRzzpXSB3zY3iZcu~buekiJCK4gN0hGMFnmkA-GLOoRR0-KW9Nek52bQlOrFpqEWr-41v4YzlIndh4Pf~s3~fkGS4oQ9g2nssETsXrlYXsTFEjMhz~5E1XGtJDKISbjRPx35KhW3Z6w~xm4yrN9ivXBQBkNN~iprMroDG4T11Qyeg4rLFw3BtwhuBpSEo0nQypQ3VdBcjWFB5sED91ZTcmOuamzdii7URpqshmtW~hXZgJ4UTT1WFMuBoBjGkxa~4s2ucyoqJqIM5ljLHZ8kbYCdzbpVIhIw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cell specificity of peptides.
Prepared human primary monocytes, neutrophils, and cultured HL60, Jurkat, U937, NIH3T3, 3Y1, 3T3L1, and PC12 cells were loaded with fura-2 and stimulated with effective concentrations of the 3 peptides (1 μM for monocytes and neutrophils; 10 μM for HL60 and Jurkat; 20 μM for U937, NIH3T3, 3Y1, 3T3L1, and PC12). (A) The peak level of the [Ca++]i increase was recorded. (B) Monocytes and neutrophils were stimulated with 10 μM of each peptide. Superoxide generation was measured using cytochrome c reduction assay. Data are presented are means ± SE from at least 3 independent experiments.
