Fig. 5.
![Fig. 5. Differentiation specificity of the peptides. / HL60 cells were cultured in RPMI medium containing 20% fetal calf serum. To induce differentiation of the cells into granulocytes, the cells were cultured in the presence 1.25% DMSO for 4 days. Healthy HL60 (■) and differentiated HL60 cells (dHL60, ▪) were loaded with fura-2 and treated with various concentrations of HFYLPM (A), MFYLPM (B), and HFYLPm (C). The peak level of the [Ca++]i increase was recorded. The data are means ± SE from 4 independent experiments. *P < .01 versus vehicle treatment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2854/6/h80910966005.jpeg?Expires=1735565412&Signature=lU0wHs5geUPl4lH7QtLDGER~yi3iNEVUehF2c31G9~9w8NTnskwKOc-jI4H7-dap9vJujCBx1qG345TzHSZBj68CXBCEdT8OkNG~VU~YgKtKGiSi17MlevKAVcKJkaS1m8D-fGTzAAgQRpelsqtG6o8ErFGMQ4C9d4-mh0TMFsXYGuu8r58cPCuoPWfPhkcgWw9as90jCZt87PMFnHHcMvVdYz5fMXwzzqqaPBUPuog3RysmWyIEVFzHLxVptW7m~QxhvLt7bn-hV3Hroex2u~-4TPrvxa7HlV4tpiKGflQvRrXzQ0wjUfHO3zVroVjbKGUrPAU37cT7aAS~Br6spQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Differentiation specificity of the peptides.
HL60 cells were cultured in RPMI medium containing 20% fetal calf serum. To induce differentiation of the cells into granulocytes, the cells were cultured in the presence 1.25% DMSO for 4 days. Healthy HL60 (■) and differentiated HL60 cells (dHL60, ▪) were loaded with fura-2 and treated with various concentrations of HFYLPM (A), MFYLPM (B), and HFYLPm (C). The peak level of the [Ca++]i increase was recorded. The data are means ± SE from 4 independent experiments. *P < .01 versus vehicle treatment.
