Chemotactic effect of peptides.

Assays were performed using a modified Boyden chamber assay as described in “Materials and methods.” Isolated human monocytes (1 × 10⁶ cells per milliliter of serum-free RPMI) were added to the upper wells of a 96-well chemotaxis chamber, and migration across the polycarbonate membrane with 5 μm pore size was assessed after 2 hours of incubation at 37°C. The number of migrated cells was determined by counting them in high-power field (400 ×). The data are presented as mean ± SE of 3 independent experiments each performed in duplicate. *P < .01 versus vehicle treatment. HFYLPM,; MFYLPM, ▪; HFYLPM, ; fMLF, ▾.