Effect of PTX on the monocyte chemotactic migration induced by the peptides.

Monocytes were preincubated with PTX (1 μg/mL) or vehicle only for 90 minutes at 37°C. The cells were used for chemotaxis assay with HFYLPM (1 μM), MFYLPM (1 μM), HFYLPm (100 nM), and fMLF (100 nM). The number of migrated cells was determined by counting them in high-power field (400 ×). The data are presented as mean ± SE of 2 independent experiments each performed in duplicate. *P < .01 versus medium treatment, †P < .01 versus peptide treatment, respectively.