Effect of enzyme inhibitors on monocyte chemotaxis induced by the peptides.
Monocytes remained untreated or were treated for 15 minutes (60 minutes for PD98059) with LY (LY294002, 50 μM), GFX (GF109203X, 5 μM), or PD (PD98059, 50 μM). After washing twice, 25 μL of monocytes at 1 × 106/mL was added to the upper wells and allowed to migrate for 2 hours at 37°C (humidified atmosphere; 5% CO2) toward each peptide (1 μM HFYLPM, 1 μM MFYLPM, or 10 nM HFYLPm) or medium. After fixing and staining of the membrane, the number of migrated cells was determined by counting them in high-power field (400 ×). The data are presented as mean ± SE of 3 independent experiments each performed in duplicate. *P < .01 versus medium treatment, †P < .01 versus peptide treatment, respectively.
