Immunophenotype of cultured and uncultured AML blasts.

Filled and unfilled histograms indicate expression of the labeled mAb and of an appropriate isotype control. Numbers indicate percentage of positive cells after subtraction of controls (mean fluorescence intensity of positive cells). (A) The immunophenotype of day-0 and day-7 cells from patient 3 (cultured with GM-CSF with TNF-α added for the last 2 days). (B) The immunophenotype of day-0 and day-7 cells from patient 14 (cultured with GM-CSF, IL-4, and TNF-α all from day 0).