Fig. 6.
Fig. 6. Modulation of STAT6 activation by IL-13 and the IL-13Rα2 chain. / CHO-K1 cells were transfected with various receptor chains and incubated with IL-13 for 10 minutes, solubilized with cold whole-cell extraction buffer, and 50 μg sample protein was incubated for 20 minutes with 1 ng 32P-labeled SBE1 probe in binding buffer. DNA-protein interaction was analyzed by SDS-PAGE analysis (A). CHO-K1 cells were transfected with increasing amounts (0 to 10.5 μg) of IL-13Rα2 cDNA along with fixed amounts of DNA for α1 IL4Rα (6 μg) chains (B) or α1 IL4Rαγc (C). For supershift assay, protein extract from α1 IL4Rα–transfected CHO-K1 cells was incubated with antihuman STAT6 rabbit polyclonal immunoglobulin G before electrophoresis (D).

Modulation of STAT6 activation by IL-13 and the IL-13Rα2 chain.

CHO-K1 cells were transfected with various receptor chains and incubated with IL-13 for 10 minutes, solubilized with cold whole-cell extraction buffer, and 50 μg sample protein was incubated for 20 minutes with 1 ng 32P-labeled SBE1 probe in binding buffer. DNA-protein interaction was analyzed by SDS-PAGE analysis (A). CHO-K1 cells were transfected with increasing amounts (0 to 10.5 μg) of IL-13Rα2 cDNA along with fixed amounts of DNA for α1 IL4Rα (6 μg) chains (B) or α1 IL4Rαγc (C). For supershift assay, protein extract from α1 IL4Rα–transfected CHO-K1 cells was incubated with antihuman STAT6 rabbit polyclonal immunoglobulin G before electrophoresis (D).

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