Characterization of MN and MPIN vectors.

(A) NGFR surface expression in the transduced NIH3T3 cells with 1:25 dilution of MN or MPIN vector supernatant. (B) Detection of the region including PIG-A in MPIN-transduced NIH3T3 cells by PCR. DNA was isolated from transduced (MN or MPIN) or untransduced (mock) NIH3T3. A region including vector plus 5′ PIG-A was amplified by PCR. For positive control, the MPIN vector plasmid was used as a template, and Marker 6 was loaded as a marker.