SDF-1α affects CXCR4 expression.

Cells were treated at day 0 after HPC purification with TPO (100 ng/mL) (thin line) or with TPO + SDF-1α (1 μg/mL) (broad line) and analyzed at intervals during the culture (days 2 through 12) by flow cytometry with the use of PE-conjugated CXCR4 mAb as described in “Materials and methods.” The staining with negative control antibody is shown, as solid profile, at day 0, and appears similar in the following days. Representative results from 1 out of 5 independent experiments are shown.