Characterization of

PU.1^−/− NK cells. (A) RT-PCR for the indicated transcripts was performed, using RNA prepared from sorted splenic NK cells (CD3⁻NK1.1⁺) fromWT and PU.1^−/− chimeras. (B) Splenic NK cells were stained with mAbs specific for the indicated surface antigens. Histogram profiles are shown for CD3⁻NK1.1⁺ gated cells. Data are representative of 8 independent experiments.