Effect of STI571 and ATRA alone or in combination on the expression of CD11b, CD11c, and CD33 myeloid markers in NB4 cells.
NB4 cells were seeded at an initial concentration of 2 × 105/mL and treated for 3 days with vehicle alone, STI571 (5 × 10−6 M), the indicated concentrations of ATRA (○), or the combination of the 2 compounds (●). The level of expression of the indicated phenotypic markers was determined by flow cytometry, using fluorescein-labeled monoclonal antibodies against CD11b, CD33, and CD11c or irrelevant isotype-matched antibodies (Neg). The percentage of marker-positive cells is shown in the upper panels, whereas the amount of cell-associated fluorescence in arbitrary units is illustrated in the lower panels. Results are the mean ± SD of 3 separate culture dishes and are representative of at least 2 independent experiments.
