Immunofluorescence assessment of H-FT levels in K562 cells.

Cells were incubated for 48 hours with 1 nM ASH-ODN (A), nothing (untreated cells, B), preimmune rabbit serum (C), and 1 nM IN-ODN sequence (D). Immunofluorescence was assayed by incubating the cells with mouse monoclonal antibodies against human H-FT. FITC-conjugated antirabbit IgG served as a secondary antibody.