Influence of iron chelation on L-FT up-modulation by H-FT repression.

DFO (0-50 μM) was added for the last 24 hours to cells incubated for 48 hours with 1 nM of either ASH-ODN (▪) or IN-ODN (○). In both the ASH-ODN (▴) and the IN-ODN (⋄), 50 μM iron-bound DFO was also supplemented. (Inset) Influence of 48-hour incubation with 1 nM ASH-ODN on the expression of both H-FT (▪) and L-FT (░) compared with the influences of DFO (50 μM, 24 hours), IN-ODN (1 nM, 48 hours), and none (48 hours). Protein levels were quantified by ELISA, as described in “Materials and methods.” Data are from a representative experiment performed in triplicate.