Electromobility shift and supershift analysis (Oct2).

The presence of Oct2 with DNA binding activity was investigated in the HD-derived cell lines KM-H2, L428, and L1236 and B-cell control cell lines Raji and Nalmalwa. No Oct2 DNA binding activity was observed in the HD-derived cell lines, whereas both control cell lines showed high DNA binding activity (left panel). Super shift analysis with Oct2-specifc antibodies (right panel) demonstrated the specificity of our technique. Note: Oct1 was present in all cell lines investigated.