![Fig. 2. RGS18 in RGS subfamily B. / (A) Phylogenetic tree of the RGS domains of all RGS family members. The primary amino acid sequences of RGS domains from 48 mammalian RGS proteins were aligned by means of Clustal W1.7. The phylogenetic tree was constructed by the neighbor-joining method by means of Clustal W1.7 on the basis of this alignment and visualized with the Treeview program 1.5. All known RGS proteins (except RGS10 and D-AKAP2) are grouped into 6 subfamilies A to F. Species abbreviations: h, Homo sapiens; m, Mus musculus; r, Rattus norvegicus; b, Bos taurus. The accession numbers for the sequences used in this analysis are as follows: hRGS18, AAF80227; hRGS1, NP_002913; mRGS1, NM_015811; hRGS2, NP_002914; mRGS2, O08849; rRGS2, AF279918; hRGS3,P49796; mRGS3, AF215670; hRGS4, P49798; mRGS4, O08899; rRGS4, P49799; hRGS5, NP_003608; mRGS5, O08850; rRGS5, NM_019341; hRGS6, NP_004287; mRGS6, AAC70011; hRGS7, AAD34290; mRGS7, O54829; rRGS7, BAA75635; bRGS7, O46470; rRGS8, BAA23680; hRGS9, AAC64040; mRGS9, O54828; rRGS9,AAC01959; hRGS10, NP_002916; hRGS11, AAC69175; hRGS12, O14924; rRGS12,O08774; hRGS13, AF030107; mRGS14, P97492; rRGS14, O08773; hRGS16,O15492; mRGS16, P97428; rRGS16, P56700; bRGS16, O46471; hRGS17,AAF08978; hAxin, AAC51624; mAxin, AAC53285; rAxin, AAC40066; bRET-RGS1,P79348; hRGS-GAIP, P49795; hRGSZ1, NP_003693; mRGSZ1, AF191554; mConductin, AAC26047; hp115-RhoGEF, NP_004697; mLsc, AAC52693; hPDZ-RhoGEF, BAA20834; mD-AKAP2, AAC61898. (B) Phylogenetic tree of all RGS subfamily B members constructed with the use of full-length amino acid sequences. (C) Alignment of RGS domains of all RGS subfamily B members. The RGS domain of mouse RGS18 is compared with that of other RGS subfamily B members. The regions of RGS domains are defined according to Tesmer et al.41 The conserved amino acids are shaded. The identical residues in all proteins in the alignment are displayed at the top. An asterisk indicates subfamily B–specific residue (S127 [single-letter amino acid code]). Residue numbers shown at the top correspond with mRGS18. Sources of primary sequences are the same as in panel A.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/10/10.1182_blood.v97.10.3051/6/h81011045002.jpeg?Expires=1735570909&Signature=WD8IVZCRGjO8GHhr7~nQ0wuAQ-NAI5NJkxT2w9WiI7jdHOjcgr-BuQnFQJNRe9VIVVheX58i8TD5o3qSem0RJThR0RNTqOIIwV7heSpL9nBNojuw0UNVjF2I22TL~8pycOsDlc6AsInYtoRGNPg4ZAGJrD1a4XfaYTWauml60ryYMD~oaa1Isw0heK0OE9EPKEWBITmPKjh6XXahm15tG4fx-0gGn0P2UvYJNUY20rXHSJ3NmbIqGkb2vZGyxnRVapbIA93i6B9kk8ZFRM5HZWBzycp8PETqkjTx5CzTrbMwHu1pzqbllbILuUVrlsCWZlyM8ToLdvimpuxTrFrQDw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
RGS18 in RGS subfamily B.
(A) Phylogenetic tree of the RGS domains of all RGS family members. The primary amino acid sequences of RGS domains from 48 mammalian RGS proteins were aligned by means of Clustal W1.7. The phylogenetic tree was constructed by the neighbor-joining method by means of Clustal W1.7 on the basis of this alignment and visualized with the Treeview program 1.5. All known RGS proteins (except RGS10 and D-AKAP2) are grouped into 6 subfamilies A to F. Species abbreviations: h, Homo sapiens; m, Mus musculus; r, Rattus norvegicus; b, Bos taurus. The accession numbers for the sequences used in this analysis are as follows: hRGS18, AAF80227; hRGS1, NP_002913; mRGS1, NM_015811; hRGS2, NP_002914; mRGS2, O08849; rRGS2, AF279918; hRGS3,P49796; mRGS3, AF215670; hRGS4, P49798; mRGS4, O08899; rRGS4, P49799; hRGS5, NP_003608; mRGS5, O08850; rRGS5, NM_019341; hRGS6, NP_004287; mRGS6, AAC70011; hRGS7, AAD34290; mRGS7, O54829; rRGS7, BAA75635; bRGS7, O46470; rRGS8, BAA23680; hRGS9, AAC64040; mRGS9, O54828; rRGS9,AAC01959; hRGS10, NP_002916; hRGS11, AAC69175; hRGS12, O14924; rRGS12,O08774; hRGS13, AF030107; mRGS14, P97492; rRGS14, O08773; hRGS16,O15492; mRGS16, P97428; rRGS16, P56700; bRGS16, O46471; hRGS17,AAF08978; hAxin, AAC51624; mAxin, AAC53285; rAxin, AAC40066; bRET-RGS1,P79348; hRGS-GAIP, P49795; hRGSZ1, NP_003693; mRGSZ1, AF191554; mConductin, AAC26047; hp115-RhoGEF, NP_004697; mLsc, AAC52693; hPDZ-RhoGEF, BAA20834; mD-AKAP2, AAC61898. (B) Phylogenetic tree of all RGS subfamily B members constructed with the use of full-length amino acid sequences. (C) Alignment of RGS domains of all RGS subfamily B members. The RGS domain of mouse RGS18 is compared with that of other RGS subfamily B members. The regions of RGS domains are defined according to Tesmer et al.41 The conserved amino acids are shaded. The identical residues in all proteins in the alignment are displayed at the top. An asterisk indicates subfamily B–specific residue (S127 [single-letter amino acid code]). Residue numbers shown at the top correspond with mRGS18. Sources of primary sequences are the same as in panel A.
