Fig. 5.
Expression and subcellular localization of RGS18 in megakaryocytes and platelets.
Fixed megakaryocytes and some hematopoietic suspension cells prepared from mouse bone marrow (A, B) and the fixed megakaryocytes derived from mouse ES cells (C, D) were stained with anti-RGS18 rat antibody followed by FITC-conjugated F(ab′)2 fragment donkey antirat, together with DAPI (B, D). RGS18 is stained in green and polyploid chromosomes in blue. The platelets isolated from mouse peripheral blood (E) and megakaryocytes during proplatelet formation (PPF) (F) were immunostained with purified FITC-labeled anti-RGS18 rat antibody. The fluorescent image of PPF (F) was overlaid on a difference-inteference contrast image.
