Fig. 6.
![Fig. 6. GAP activity of RGS18 for specific Gα subunits. / Different concentrations of recombinant RGS18 or recombinant RGS4 were tested for their ability to accelerate the GTPase activity of Gαi (A), GαqR183C (B), Gαs (C), and Gα12 (D). Hydrolysis of GTP was initiated by adding the Gα subunit loaded with γ-32P]GTP to a buffer containing MgSO4 with the indicated amount of RGS protein. The reaction mixture was incubated, and aliquots were removed at the indicated times and processed, and the amount of 32Pi was counted by liquid scintillation spectrometry. HED is buffer without RGS protein. The GAP assays were performed twice with similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/10/10.1182_blood.v97.10.3051/6/h81011045006.jpeg?Expires=1735568916&Signature=dyrqgyvAFbGTCnGfan4u~Qjg~7~bMJ98wnUGkLdXlM9IRPmt9YxXJQV0KmNySbWKPJZBycOQB2F8MhWIztTmG75KA-cmR0i90b0pH7fdffykcBdnV95kCIhdEKzAsdEDglnDU8VumLxcByqBjZzD4s9lIUrT0kDEOoGUiuAOlIJiDdenm~YGqP1XqBpfD9Dx-0esrRZxayanCrcj7u5PZxnneBewDMx7mldkuMfRTvaPYmftBCzfBM0wez0glyXJjOk3DQmwr4ImKgW0rXlOZgBG0TB0PtBm2eSAVS37TTiRgmmGM63wgi1yIxOCNHEb3EDbAdZaHTDkVxm7OH81tQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
GAP activity of RGS18 for specific Gα subunits.
Different concentrations of recombinant RGS18 or recombinant RGS4 were tested for their ability to accelerate the GTPase activity of Gαi (A), GαqR183C (B), Gαs (C), and Gα12 (D). Hydrolysis of GTP was initiated by adding the Gα subunit loaded with γ-32P]GTP to a buffer containing MgSO4 with the indicated amount of RGS protein. The reaction mixture was incubated, and aliquots were removed at the indicated times and processed, and the amount of 32Pi was counted by liquid scintillation spectrometry. HED is buffer without RGS protein. The GAP assays were performed twice with similar results.
