Fig. 7.
RGS18 binds to Gαi and Gαq in megakaryocytes.
ES cell–derived primary megakaryocytes (A) or FD-TPO cells (B) were treated with GDP (lanes 2 and 5) or GDP and AlF4− (lanes 3 and 6), and the cell lysates were incubated with GST-RGS18 immobilized on glutathione Sepharose beads. Bound proteins were separated by 10% SDS-PAGE and detected with anti–Gαi-3 antibody or anti-Gαq/11 antibody. Arrowheads indicate the bound Gα of 42 kd. Lanes 1 and 4 show the immunoblots of total cell lysates.
