Fever-range WBH augments the ability of PLN HEV to support L-selectin–dependent lymphocyte adhesion.

(A) PLN from BALB/c mice treated for 6 hours with WBH (core temperature, 39.8 ± 0.2°C) and normothermic controls (36.9 ± 0.2°C) were used in frozen-section adhesion assays. Arrows indicate HEV structures containing darkly stained adherent human PBL indicator cells; bar indicates 50 μm. (B) Quantification of human PBL adhesion to PLN HEV from normothermic mice and from mice treated for 2 or 6 hours with WBH. Before assay, PBL or PLN tissue sections were incubated for 30 minutes with the indicated function-inhibiting mAb. * indicates P < .0008; **, P < .02. (C) Treatment groups included normothermic controls, mice treated for 6 hours with WBH, and mice treated for 6 hours with WBH and then maintained at room temperature for an additional 12 hours to allow core temperatures to return to normal (37.0 ± 0.2°C). PLN were removed and used in frozen-section adhesion assays to evaluate human PBL adhesion to HEV. * indicates P < .002. (D) Adhesion of murine 300.19/L-selectin transfectants and 300.19/L-Δcyto cells was evaluated in frozen sections of LN HEV from normothermic mice and mice treated with WBH for 6 hours. * indicates P < .0007. The differences between HEV adhesion in PLN from normothermic mice and WBH-treated mice were significant by unpaired 2-tailed Student ttest.