Increased PLN HEV adhesion is associated with febrile temperatures after WBH treatment or inflammation induced by turpentine or LPS.

The core temperature of mice was raised to the indicated temperature by either WBH treatment for 6 hours (left panel), injection of turpentine oil ([Turp], 100 μL given subcutaneously; middle panel), or injection of LPS (10 μg/kg given intraperitoneally; right panel). Control mice were given injections of sterile saline. PLN tissues were isolated at the indicated times, and adhesion of human PBL to HEV of frozen sections was quantified under shear conditions. Before assay, selected PBL samples were pretreated with DREG-56 blocking mAb. Data are the mean ± SD results with triplicate samples; results are representative of 3 independent experiments. The differences between HEV adhesion in PLN from normothermic mice and those from mice at febrile temperatures were significant by unpaired 2-tailed Student t test. * indicates P < .0001; ▪, untreated; and ■, DREG-56 mAb treated.