Identification of a domain responsible for repression in Evi-1.

(A) Structures of wild-type Evi-1 and its deletion mutants. The Smad3-binding domain and the repression domain are shown. (B) HepG2 cells were transfected with p3TP-Lux together with wild-type or each mutant of Evi-1 in the absence or the presence of 200 pM TGF-β as indicated. (C) HepG2 cells were transfected with Smad3, Smad4, and p3TP-Lux together with wild-type or each mutant of Evi-1 as indicated in the absence of TGF-β. Luciferase activities were measured, and the values relative to the basal activity of the reporter are presented. The representative data of 3 independent experiments in duplicate are shown. Values and error bars depict the mean and the SD, respectively.