The whole structure of CtBP is required for interacting with Evi-1.

(A) Schematic presentation of GST-CtBP FL, GST-CtBP (1-330), GST-CtBP (111-440), GST-CtBP (1-220), and GST-CtBP (221-440). (B) pEBG (lane 1), CtBP FL (lane 2), CtBP (1-330) (lane 3), CtBP (111-440) (lane 4), CtBP (1-220) (lane 5), or CtBP (221-440) (lane 6) in pEBG was transfected into COS7 cells (4 × 10⁶) together with Evi-1 in pME18S. Cell extracts were incubated with Glutathione Sepharose beads and washed extensively, and GST-CtBP–bound Evi-1 was detected by Western blot by means of anti–Evi-1 (top). Expression of GST-CtBP fusion constructs and Evi-1 is monitored with anti-GST (middle) and anti–Evi-1 (bottom), respectively. Positions of size markers (in kilodaltons) are indicated on the left.