Fig. 4.
![Fig. 4. Evi-1 interacts with CtBP1 in vitro. / (A) Construction of GST fusion proteins of the wild-type or mutant forms of Evi-1 (544-607). (B) GST pull-down experiments. Here, 5 μg bacterially expressed GST alone (lane 1), GST-DL/DL (lane 2), GST-AS/DL (lane 3), GST-DL/AS (lane 4), and GST-AS/AS (lane 5) were incubated with in vitro–translated [35S]methionine-labeled CtBP1. After extensive washing, the samples were subjected to SDS-PAGE followed by autoradiography (top). Half of the input of in vitro–translated [35S]methionine-labeled CtBP was presented in lane 6. Positions of size markers (in kilodaltons) are indicated on the left. Coomassie stains of GST-fusion proteins are indicated (bottom).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2815/6/h80910983004.jpeg?Expires=1735825006&Signature=t4JCu3VL2h0wrTb0o0O9HluBrs4WG1AcebZA8Znj4Km0WXNYxYtI8LkfLMDLxaP3v3bBbfjZ~Cc90CWq0CFxgd4gKSgUIjykITtKYqSOVteRmVV68leFq3FFePDr0sVFH8HQV8VTCZZM5zvRMLorMYJqREKuZ0j3tvh2HnQVG5kTq58uaoo8eME~vI4fECl0RGSM1whhwDEiNO8lb8esk94wtzgAGSPNrSZrZnxNRRQjQvticOlLqLFRoVvcYNtezdTtCkxEvD2GFe9X9ZHjrnjmvDm7R3k7YJChblLNtMa3RNtVL4lNHmisZtIDpRzqrznn6HtkS3-eT37fNFJfvA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Evi-1 interacts with CtBP1 in vitro.
(A) Construction of GST fusion proteins of the wild-type or mutant forms of Evi-1 (544-607). (B) GST pull-down experiments. Here, 5 μg bacterially expressed GST alone (lane 1), GST-DL/DL (lane 2), GST-AS/DL (lane 3), GST-DL/AS (lane 4), and GST-AS/AS (lane 5) were incubated with in vitro–translated [35S]methionine-labeled CtBP1. After extensive washing, the samples were subjected to SDS-PAGE followed by autoradiography (top). Half of the input of in vitro–translated [35S]methionine-labeled CtBP was presented in lane 6. Positions of size markers (in kilodaltons) are indicated on the left. Coomassie stains of GST-fusion proteins are indicated (bottom).
