Effect of trichostatin A on Evi-1–mediated repression.

HepG2 cells were transfected with p3TP-Lux together with wild-type or each mutant of Evi-1 in the presence of 200 pM TGF-β as indicated. Luciferase activities were measured 48 hours after transfection following an 8-hour treatment with (▪) or without (■) 50 ng/mL trichostatin A. Luciferase activities relative to the basal activity of the reporter are presented. Values and error bars depict the mean and the SD, respectively.