![Fig. 7. Association with CtBP is required for Evi-1 to block TGF-β–induced growth inhibition. / (A) Expression of wild-type Evi-1 and its mutant form, DL/AS, in the stable Mv1Lu transfectant clones. Clones W-55 (lane 3) and W-60 (lane 4) were established from cells transfected with wild-type Evi-1, while A-72 (lane 5) and A-82 (lane 6) were from cells transfected with the DL/AS mutant of Evi-1. M-2 (lane 1) and M-4 (lane 2) are mock clones transfected with pME18Sneo empty vector. The results of immunoblotting with anti–Evi-1 are shown. (B) The Mv1Lu clones stably transfected with wild-type Evi-1 (W-55, ▵, and W-60, ▴), DL/AS (A-72, ■, and A-82, ▪), and control clones (M-2, ○, and M-4, ●) were subjected to a [3H]thymidine-incorporation assay in the presence of the indicated concentrations of TGF-β. Results are expressed as percentages relative to values obtained from control cultures that did not receive TGF-β. Values and error bars depict the mean and the SD, respectively.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2815/6/h80910983007.jpeg?Expires=1735825433&Signature=SBUrGcMaQHVHRbR~-evu5P3736eJJPgbUbJlRUjAGU2DgKWMpZTIYh1cVx-2znGcGZ~q5arfPtkKt344RqViyXnreNcrD2aRnT3Eg36IBqemhK4yM8YND-MrzBtjH2rXkIaQBXzo~NgVf9WDimsK-wv7yCUrbzkCYwaDi35IWkO1GyWsafcX2ICI16PHercWHuXFCBfQqXIVgDsMtUi1Septm8hHxoTpfuMEpvG5zlSqmnNkk4uvqZcbstL~BOORa04mviYnSHkRMwK~hkUGswWapAePBC2pVjbvI98jxb1HFAjN5pMHhrVN1k25SVj5HHs1jb4CI3H8KIo3-2H9nA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Association with CtBP is required for Evi-1 to block TGF-β–induced growth inhibition.
(A) Expression of wild-type Evi-1 and its mutant form, DL/AS, in the stable Mv1Lu transfectant clones. Clones W-55 (lane 3) and W-60 (lane 4) were established from cells transfected with wild-type Evi-1, while A-72 (lane 5) and A-82 (lane 6) were from cells transfected with the DL/AS mutant of Evi-1. M-2 (lane 1) and M-4 (lane 2) are mock clones transfected with pME18Sneo empty vector. The results of immunoblotting with anti–Evi-1 are shown. (B) The Mv1Lu clones stably transfected with wild-type Evi-1 (W-55, ▵, and W-60, ▴), DL/AS (A-72, ■, and A-82, ▪), and control clones (M-2, ○, and M-4, ●) were subjected to a [3H]thymidine-incorporation assay in the presence of the indicated concentrations of TGF-β. Results are expressed as percentages relative to values obtained from control cultures that did not receive TGF-β. Values and error bars depict the mean and the SD, respectively.
