Effect of polymyxin B and sTNFR-p55 on induction of MIP-1α and MIP-1β mRNAs.
(A) Nonadherent human monocytes (7 × 106 cells) were left untreated or stimulated for 1 hour at 37°C with either anti-CD11b (5 μg/mL), anti-CD11c (5 μg/mL), LPS (200 ng/mL), ZZ-CD23 (1 μg/mL), or MBP-CD23 (1 μg/mL) in the presence or absence of polymyxin B (10 μg/mL). (B) Monocytes (7 × 106cells) were left untreated or cultured for 1 hour at 37°C with anti-CD11a (5 μg/mL), anti-CD11b (5 μg/mL), anti-CD11c (5 μg/mL), MBP-CD23 (1 μg/mL), ZZ-CD23 (1 μg/mL), or soluble TNF-α (10 ng/mL) in the presence or absence of sTNFR-p55 (10−8 M). RNA was isolated and analyzed by RPA for expression of MIP-1α, MIP-1β, IL-8, and GAPDH mRNAs. Results are representative of 2 distinct experiments.
