Fig. 6.
Ligation of β2 integrins induces IκB-α degradation.
Nonadherent monocytes were preincubated for 30 minutes with or without 10 μg/mL cycloheximide and then stimulated for various times with anti-CD11b mAb (10 μg/mL; A), anti-CD11c mAb (10 μg/mL; B), MBP-CD23 (1 μg/mL; C) or ZZ-CD23 (5 μg/mL; D). Whole-cell extracts were prepared and 50 μg protein was separated on 10% SDS-PAGE and transferred to nitrocellulose membranes. IκB-α proteins were revealed with anti–IκB-α polyclonal serum followed by an ECL reaction. Diagrams represent the densitometric scanning of IκB-α protein levels in each Western blot. Results are representative of 2 experiments.
