Enhancing effect of RBCs on T-cell expansion does not require monocytes.

PBLs and T cells were stimulated in 96-well plates and cultured for 5 days in the absence or presence of RBCs. Enriched monocytes (Mo, more than 70% CD14⁺) were added to the cultures at monocyte:PBL/T-cell ratios of 1:8 and 1:1. Results show thymidine uptake (mean ± SD of triplicates) in the different culture conditions. One representative of 3 separate experiments is shown.