Induction of

Cyr61 and CTGF mRNA by Xa/hirudin. (A) Time dependence. Total RNA from serum-starved HeLa cells was purified after the indicated periods of stimulation in the presence of 50 nM thrombin or 50 nM Xa/100 nM hirudin. Transcripts forCyr61, CTGF, and GAPDH (G3) as a loading control were detected by Northern blotting. (B) Dose dependence. Cells were preincubated with 100 nM hirudin for 2 minutes, followed by the addition of the indicated concentrations of Xa for 90 minutes. Transcripts for Cyr61, CTGF, andG3 were detected by Northern blotting.