Fig. 1.
![Fig. 1. TMT and FcεRI expression. / Nonsorted, freshly isolated cells from asthma patient 1 (A,B) and asthma patient 2 (C-E) were stained with antihuman TMT IgG alone (A,C). Cells from patient 1 were also sequentially stained with anti-TMT IgG (brown) and then with an antibody that recognizes human tryptases α, βI, βII, and βIII (pink) (B). Cells from patient 2 were sequentially stained with anti-TMT IgG (brown [D] or pink [E]) and then with either antichymase IgG (pink) (D) or anti-FcεRI IgG (brown) (E). Note that the metachromatic cells in the blood of both patients express a number of the granule proteases that normal, mature MCs express, but nearly all of these cells possess segmented nuclei like normal blood basophils. Magnification ∼×400.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/11/10.1182_blood.v97.11.3484/6/h81111135001.jpeg?Expires=1735488075&Signature=BB1VK59ZlhSzrc18rwHWWojWHOpfRHQnbPDuv1Yfs8SpTjwVqHcgVSIpIBr-YrN0dFxU-PTw9Stx5gv6uBDb5WfRHAgsJvExOPXA5mKomkZo5vCatzh0GUb15VNVe3hFtSPhZTJdLNJxhU82e6fZrYsUIIj0t4LvIzu7W021JplHLNHmn7K-Ylr3bYbC8rEFVwqVj47tLEGfF5QhyIzRocOKYuTqZVJbzWK5xMWdhFEuiDUnVsspS3dAnKEuX2jP69SXRnxyAoh1T8Vhs09vGkYV3SklQus3UD2rxyz-p9h5IODKx1xqAkjcIvEi0n08mhOX-Q9wYS62u1uG63j2OQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TMT and FcεRI expression.
Nonsorted, freshly isolated cells from asthma patient 1 (A,B) and asthma patient 2 (C-E) were stained with antihuman TMT IgG alone (A,C). Cells from patient 1 were also sequentially stained with anti-TMT IgG (brown) and then with an antibody that recognizes human tryptases α, βI, βII, and βIII (pink) (B). Cells from patient 2 were sequentially stained with anti-TMT IgG (brown [D] or pink [E]) and then with either antichymase IgG (pink) (D) or anti-FcεRI IgG (brown) (E). Note that the metachromatic cells in the blood of both patients express a number of the granule proteases that normal, mature MCs express, but nearly all of these cells possess segmented nuclei like normal blood basophils. Magnification ∼×400.
