Dose-dependent expression of PF-8 and gpK8.1 in TPA-stimulated BCBL-1 cells in normoxia and hypoxia (acute and chronic exposure).
BCBL-1 cells that had been cultured in normoxia (21% O2) or hypoxia (1% O2) were seeded at 300 000 viable cells per milliliter and incubated overnight at the indicated conditions. Following the overnight incubation, the cells were treated with increasing concentrations of TPA and maintained at the indicated conditions following TPA treatment for 48 hours. In each panel, the values represent 1 of 2 similar experiments. (A) PF-8 expression in BCBL-1 cells in normoxia (▪) or acute hypoxia (●). (B) gpK8.1 expression in BCBL-1 cells in normoxia (▪) or acute hypoxia (●). (C) PF-8 expression in BCBL-1 cells in normoxia (▪) or chronic hypoxia (●). (D) gpK8.1 expression in BCBL-1 cells in normoxia (▪) or chronic hypoxia (●).
