Fig. 2.
FPR activation suppresses mRNA.
Activation of FPR by fMLP or T-20 suppresses the level of mRNA for both IL-12 p35 (A) and IL-12 p40 (B). Human monocytes were stimulated for 6 hours with SAC and IFN-γ in the presence or absence of FPR and FPR-1 ligands (10−5 M). Taqman was used to isolate and quantitate RNA. Suppression of IL-12 mRNA was expressed as percentage maximal induction relative to peak expression with SAC and IFN-γ stimulation because there was no detectable constitutive expression of IL-12 p40 mRNA. Data are mean ± 1 SD from replicate samples from 3 separate donors. All samples were normalized for GADPH expression performed concurrently with IL-12 mRNA amplification. ND, not detected.
