Neither fMLP, T-20 peptide, nor F-peptide suppresses IL-12 p70 production by monocyte-derived dendritic cells.

Immature dendritic cells (CD1a^high/CD83^low) were generated from human monocytes cultured for 7 to 9 days in GM-CSF and IL-4 and then were stimulated with IFN-γ and either SAC (■) or CD40L (▨) in the presence or absence of fMLP (10⁻⁵ M), T-20 (10⁻⁵ M), or F-peptide (10⁻⁵ M). IL-12 p70 concentrations were measured 24 hours after stimulation by ELISA. Data are mean ± 1 SD from dendritic cells derived from 3 separate donors (all P > .05).