Fig. 6.
Immature dendritic cells have functional expression of FPR but not of FPRL-1.
Dendritic cells were loaded with the calcium fluorochrome Indo-1, and intracellular calcium flux by CD1a+ cells was assayed by FACS analysis. Dendritic cells were stimulated with 10−5 M fMLP, 10−5 M T-20 peptide, or 10−5 M F-peptide followed by 10−8 M fMLP. Calcium flux is expressed as the ratio of bound to free intracellular calcium, and scattergrams represent changes in ratios of bound to free calcium for the gated CD1a+ population. FACS data are representative of 2 independent experiments yielding similar results.
