Histologic findings in tibia and femur of a MRL/lpr mouse 14 days after treatment with 5.5 Gy × 2 + IBM or 5.5 Gy × 2 + IV.

The tibia (directly injected with BMCs, A) and femur (not injected, B) of the MRL/lpr mouse treated with 5.5 Gy × 2 + IBM show hyperplastic bone marrow 14 days after the treatment. In contrast, the tibia (C) and femur (D) of the mouse treated with 5.5 Gy × 2 + IV show hypoplastic bone marrow 14 days after the treatment. (E) Immunohistochemical analysis of bone marrow treated with 5.5 Gy × 2 + IBM. The frozen specimen of the bone marrow from the tibia was stained with anti-PA6 mAb followed by PE-antirat IgM and further stained with FITC-anti-H-2D^b mAb. The stained samples were examined on a confocal laser scanning microscope. Note that there is a scattering of cells stained both with anti-PA6 mAb and anti-H-2D^b mAb (yellow-colored). Donor-derived BMCs are stained green.