Fig. 5.
Generation of donor-derived stromal cell.
The bone pieces without BMCs from MRL/lpr mice 10 days after the treatment with 5.5 Gy × 2 + IBM were cultured for 3 weeks, and the cultured stromal cells were then collected. The cells were stained with anti-PA6 mAb followed by PE-anti-rat IgM, then blocked with normal rat IgM. They were further stained with FITC-anti-H-2Db mAb. Quadrants in the figures were set by the staining profile of the cells treated with isotype-matched Ig controls.
