Analysis of freshly purified PMN purity by FcγRI gene expression.

PMNs were isolated from a buffy coat and incubated overnight in the presence or absence of IFNγ as indicated. NK and monocytes were purified from freshly isolated PBMCs by magnetic cell sorting. With the use of specific primers, we analyzed FcγRI, FcγRII, and β-actin mRNA expression by RT-PCR as described in “Materials and methods.” The present data are given in the following order: Markers (M), β-actin (lanes 1, 4, 7, and 10), FcγRI (lanes 2, 5, 8, and 11), and FcγRII (lanes 3, 6, 9, and 12).